Systematic dissection and trajectory-scanning mutagenesis of the molecular interface that ensures specificity of two-component signaling pathways.
Two-component signal transduction systems enable bacteria to sense and respond to a wide range of environmental stimuli.Sensor histidine kinases transmit signals to their cognate response regulators via phosphorylation.The faithful transmission of information through two-component pathways and the avoidance of unwanted cross-talk require exquisite